The multiclamp 700b microelectrode amplifier is a versatile, computercontrolled microelectrode amplifier designed for patch voltageclamp resistivefeedback, four ranges or highspeed current clamp voltagefollower, three ranges recording within the same headstage. Multiclamp 700b axoclamp 900a 2012 for research use only. Contact your local service provider help line 180032890. Request pdf chemosensory modulation of neural circuits for sodium appetite. Analog bar displays rapidly changing input graph signals. Axon multiclamp microelectrode amplifier manual download. This versatile amplifier is capable of singlechannel and wholecell voltage patch clamp, highspeed current clamp sharp electrode or field potentials, ionselective. Manual clamp accessories dimensions and technical information are subject to change without notice clamping technology 8. Replacement manuals are available upon request at no charge at operation. The multiclamp 700b commander program runs on the us windows xp2000me98 operating systems, as well as macintosh os x, version 10. The use of any hoist presents some risk of personal injury or property damage. Can anybody help me to fix this problem with multiclamp 700b.
The voltage at the parts marked with this symbol may be dangerous. Multiclamp 700b microelectrode amplifier molecular devices. Our products include highdensity polyethylene electrofusion pipe fittings, electrofusion processors, hot tapping and plugging equipment, pipe repair products, and custom fabricated steel meter and regulator stations. Buzz variable duration train assists cell penetration. The partly opened handle is used for individual mounting of the multiclamp and as a support. Amplifier axon multiclamp 700a theory and operation 159 pages computercontrolled microelectrode amplifier amplifier axon digidata 22a operators manual 32 pages. Multiclamp 700b microelectrode amplifier kf technology. This manual has been compiled with the assumption that the sb700 will be used in combination with a camera compatible with cls and a. Axon multiclamp 700b manuals manuals and user guides for axon multiclamp 700b. Detect the presence of voltage without contacting the circuit.
Patchclamp amplifier multiclamp 700b molecular devices. If commander opens in demo mode reported in the title bar, you will need to connect the amplifier to the software. Axon multiclamp 700a theory and operation 159 pages computercontrolled microelectrode amplifier. Warning shipping the axopatch 200b the axopatch 200b is a solidly built instrument designed to survive shipping around the world. I am using it for patch clamp in hippocampal neurons.
Operation manual test leads 9v battery carrying case holster 3. For more information, visit our website or call axon technical support. Provide the customer support coordinator with the modelmachine name, machine type, and serial number that are located on the identification plate. Bnc2090a user manual rackmount connector accessory for em series daq devices bnc2090a user manual january 2007 372101a01. In order to make full use of the meters functions and ensure safe operation, please carefully read and follow the procedures in the operation manual. Also if i set the holding voltage to say a set value of 60mv then for that setting of the amplifier the potential that we. Sources such as handheld radio transceivers, radio and tv transmitters, vehicle radios and.
Axopatch 200b when handling the headstage input since this will upset the thermal equilibrium. Internal automatic mode switch instantly switch recording modes by using the membrane potential as the trigger. Even though this instrument complies with the immunity standards, its accuracy can be affected by strong radio emissions not covered in the above standards. This operation manual contains important information for. Axon multiclamp 700b theory and operation pdf download. The softpanel is essentially a hardware extension of the multiclamp 700b commander software. Preset a voltage threshold in currentclamp mode, and multicclamp700b will automatically switch to voltageclamp when membrane potential reaches that voltage level. Newcomers to electrophysiology will gain an appreciation of the intricacies of electrophysiological measurements and the requirements for setting up a complete recording and analysis system. We have 1 axon multiclamp 700b manual available for free pdf download. Pin clamp cylinder mounting variations clkqglclkqpl series series body shape symbol dimension mounting mounting hole tap, pin hole arrangement mounting surface viewed from top page symbol port location clkqg builtin standard magnet clkqp builtin strong magnet d l66 mounting tap. Computercontrolled dual channel resistivefeedback patch clamp and highspeed current clamp amplifier the multiclamp 700b is useful for a wide variety of intracellular or extracellular recording, including. Deason company is a united states manufacturer of natural gas pipeline, metering, regulation, and distribution products. View and download axon multiclamp 700b theory and operation online. The instructions given in this manual must be interpreted accordinglyand sound judgment used in determining their application.
Trademarks mentioned herein are property of molecular devices, llc or their respective owners. We have 1 axon multiclamp 700a manual available for free pdf download. Oscillation suppression sudden changes in membrane or pipette parameters may result in undesirable oscillations during whole cell recording. No part of this manual may be reproduced, stored in a retrieval system, or transmitted, in any form or. Digital clamp meter operation manual introduction clamp meter is 3 12 digit lcd and standard 9v battery operation for measuring dc voltage, ac and dc current, resistance and continuity test. If the display indicates one or more leading zeros. Thus, no special skills are required to read this manual. Clamping around more than one wire will result in erroneous readings. It is matching with 500v insulation test attachment for insulation test function. Some functions are common to both voltage and current clamp. Powerclamp series 4 hybrid multistaged high energy transient voltage surge suppressor powerclamp is a sophisticated surge suppression unit that provides the ultimate in transient protection with much lower clamping levels than any other tvss device. For complete information and specifications, see the multiclamp 700b manual.
Although this manual has been prepared with ever y precaution to ensure accuracy, mds analytical technologies assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. The 270 measures the current by clamping the jaw around one conductor wire. Axon multiclamp 700a microelectrode amplifier axon instruments multiclamp 700a is a versatile computercontrolled amplifier capable of singlechannel, wholecell and ionselective electrode recordings, as well as voltammetry and amperommetry. This operation manual is intended to help the operator to become familiar with elephant lifting products girder clamps and how to use them properly. Switch modes conditionally instantly switch recording modes by using the membrane potential as the trigger. The multiclamp 700b amplifier from molecular devices is a computercontrolled microelectrode current and voltage clamp amplifier for electrophysiology and electrochemistry. Multiclamp 700b manuals and user guides for axon multiclamp 700b. Knobs and buttons replace mouse or keyboard control. A usb port is required to connect the multiclamp 700b.
Purpose of this manual this instruction manual is intended to familiarize all personnel with the safe operation and maintenance procedures for the greenlee 77mg, 77hpg, and 77hp g6a tone generators. The bionic multiclamp is used for a simple and gentle clamping of different tubings. Multiclampmeter pro reliable and fast current measurement in all closed current circuits measures direct and alternating currents with true r. Do not attempt to make a current measurement with the test leads. Multiclamp 700b microelectrode amplifier user guide. Instructions and parts list important safety information. Multiclamp 700b university of california, san diego. Usb stick supplied with product user manual and dataview software. Measur ement category iii 300v, pollution degree 2, measurement category ii 600v, pollution degree 2, data hold switch for easy reading in. Preserve membrane potential slight voltage drift often due to changing electrode properties may contaminate an otherwise decent currentclamp recording. Earth ground double insulation protection class battery general description threephase digital power clamp meter is a handheld aptitude meter with power measurement, it is incorporated current meter and power measurement instrument. Powerclamp series 4 hybrid multistaged high energy transient voltage surge suppressor technical specifications powerclamp is a sophisticated surge suppression unit that offers the ultimate in transient protection. In current clamp mode, but with i0, i use a gain, and feedback resistor rf500 megaohm.
Multiclamp 700b amplifier the multiclamp 700b microelectrode amplifier is a versatile, computercontrolled microelectrode amplifier designed for patch voltageclamp resistivefeedback, four ranges or highspeed current clamp voltagefollower, three ranges recording within the same headstage. Included with each machine is an instructions and parts list manual. Grippa heavy duty beam clamp manual ergonomic partners. Hi all, i am seeking for your help with the multiclamp 700b. Molecular devices multiclamp 700b amplifier automate. The multiclamp 700b is the successor to the multiclamp 700a. Features safety design conforming to the following provisions of iec61010.
Molecular devices multiclamp 700b amplifier automate scientific. To disable auto off, hold down the range button while turning on the 265. Axon multiclamp 700b computercontrolled current and patch clamp amplifier for sale. Firmly closed luerlock connections are loosened fast and effortless. And sometimes when i did pipette offset, the holding voltage just suddenly.
Tubings can also be fixed safely and easily with this multifunctional scissor clamp. Oscillation suppression sudden changes in membrane or pipette parameters may result in. Copyright 2005 axon instruments molecular devices corp. Measur ement category iii 300v, pollution degree 2, measurement category ii 600v, pollution degree 2, data hold switch for easy reading in dimly light or hardtoread locations. Multiclamp 700b highspeed current clamp molecular devices. Follow all instructions and warnings for inspecting, maintaining and operating this hoist. Technical assistance replacement parts and additional manuals. The multiclamp 700b microelectrode amplifier offers highquality voltage and currentclamp capability with fast mode switching and oscillation suppression, all under convenient computer control. Together with its extensive set of signal conditioning features, the multiclamp 700b amplifier is the choice for a large variety of experimental needs. The meter meets gbt 97892 digital multimeter general technology conditions, gb4793. I am not able to set the amplifier settings for the multiclamp 700b commander in order to get the exact holding voltage that i am entering. Axon multiclamp 700b computercontrolled current and patch. Set of 2, colorcoded silicone test leads, test probes and alligator clips.
Chemosensory modulation of neural circuits for sodium appetite. It is assumed that you already have a basic understanding of technical and software terms. Powerflex 700 ac drives frames 010 user manual rockwell. Auto off automatically powers off after 10 minutes of inactivity. Before we begin, switch on your multiclamp 700b and open multiclamp commander. Diagnostics following the manufacturers instructions. Userspecified delay will allow you to perform creative new experiments. However, in order to avoid damage during shipping, the axopatch 200b must be properly packaged.
Study manual study manual again ask someone who uses pclamp clampex tutorial online help knowledge base webinar. Preset a voltage threshold in currentclamp mode, and multiclamp 700b will automatically switch to voltageclamp when the membrane. Features and benefits agency meets ce and iec 1010. Overload problem in multiclamp 700b scientist solutions. Shift to the 200a range to improve the resolution of the measurement. An additional usb port is required if the optional softpanel is used to control the amplifier. Important safety information, refer to the operating manual dangerous voltage may be present. Overload problem in multiclamp 700b i am using multiclamp 700b and pclamp10 for my extracellular recording experiments. The axon guide third edition is a tool benefiting both the novice and the expert electrophysiologist. Download pdf versions of our multiclamp amplifier manuals. Also if i set the holding voltage to say a set value of 60mv then for that setting of the amplifier the potential that we want to set gets multiplied by a factor of 10 or so. Seal test with variable amplitude and frequency fastslow electrode capacitance compensation automatic manual whole cell capacitance compensation automatic manual. Highspeed current clamp sharpelectrode or field potentials patch clamp wholecell, macropatch or excised patch voltammetry amperometry ionselective measurement bilayer recording.
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